What is spectophotometry? What is its application?

 Spectrophotometry is a analytical technique that involves the use of a spectrophotometer, a device that measures the intensity of light as it passes through a sample, to determine the concentration of a particular substance in a solution.

Spectrophotometry works by measuring the amount of light that is absorbed or transmitted by a sample at a particular wavelength. The absorption or transmission of light by a sample is influenced by the presence of certain substances, and by measuring the amount of light absorbed or transmitted at different wavelengths, it is possible to determine the concentration of those substances in the sample.

Spectrophotometry has a wide range of applications in various fields, including chemistry, biology, and medicine. It is often used to measure the concentration of specific substances in a solution, such as enzymes, proteins, and other biomolecules. Spectrophotometry is also used to analyze the purity of substances and to identify unknown substances. In addition, spectrophotometry is used in environmental testing to measure the concentration of pollutants in water, soil, and air.